LOC_ABRAMSON-Abramson Building Req ID: 82162
Employment Status: Regular – Full Time
Dr. Ruffner is a pediatric allergist whose goal is to improve to care of patients with food allergy syndromes by understanding how the immune system’s interaction with food antigens and the mucosal surfaces are altered in patients with non-IgE mediated food allergies. The Ruffner laboratory studies the genetic and immunologic mechanism that underlie non-IgE mediated food allergies such as eosinophilic esophagitis (EoE) and food-protein induced enterocolitis syndrome (FPIES). The mechanism of non-IgE mediated food allergies are relatively poorly understood compared to food allergen anaphylaxis, which is mediated by IgE. The mission of our research is to improve treatment for patients with these allergies by improving the understanding of the immunologic and genetic causes of these allergic disorders.
Our lab uses tissue culture models, immunologic assays and multi-omics approaches to study human allergic disorders.
In close collaboration with Dr. Ruffner, the research technician will play a vital role in the ongoing daily activities of the laboratory. The successful candidate for this position will be well-organized, intellectually curious, and motivated to both learn new techniques and/or apply any skills they may have already developed to our work. Prior experience with one or more of the following: aseptic tissue culture techniques, flow cytometry, and nucleic acid extraction/polymerase chain reaction are strongly preferred but not required.
- Performs routine tests under the direction of principal investigator or supervisor
- Performs research procedures on experimental samples as directed
- Records and checks results for completeness and accuracy
- Performs calculations and maintains appropriate log and lab notebooks
- Performs tasks that require familiarity with fundamental techniques and equipment
- Assists with planning specific research protocols and procedures
- Maintains office, lab area, reagents, supplies and equipment
- Cleans and performs routine maintenance on lab equipment
- Coordinates schedules of tests & procedures
- Attends courses, seminars, and lab meetings to expand knowledge of research and laboratory techniques
- Records results, organizes data and maintains lab notebook in an organized manner
- Conducts literary searches as directed
Job Responsibilities (Continued)
Job Responsibilities (Continued)
Required Licenses, Certifications, Registrations
Required Education and Experience
Required Education: Associates Degree or active enrollment in a Bachelor’s Degree Program
Required Experience: Nine (9) months of laboratory experience
Preferred Education, Experience & Cert/Lic
Preferred Education: Bachelor’s Degree
- Eighteen (18) months of laboratory experience
- Related internship experience.
Additional Technical Requirements
All CHOP employees who work in a patient building or who provide patient care are required to receive an annual influenza vaccine unless they are granted a medical or religious exemption.
Children’s Hospital of Philadelphia is committed to providing a safe and healthy environment for its patients, family members, visitors and employees. In an effort to achieve this goal, employment at Children’s Hospital of Philadelphia, other than for positions with regularly scheduled hours in New Jersey, is contingent upon an attestation that the job applicant does not use tobacco products.
Children’s Hospital of Philadelphia is an equal opportunity employer. We do not discriminate on the basis of race, color, gender, gender identity, sexual orientation, age, religion, national or ethnic origin, disability or protected veteran status.
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